Microscopy Lab - TEM Course Outline
May 15 |
Week 1 -*Fixation, dehydration and embedding in plastic. |
May 22 |
Week 2 -*From blocks to staining. Mounting and trimming samples, making glass knives and introduction to the microtome. |
May 30 |
Week 3 -Making thick sections, staining for light microscopy, light microscope photography. |
June 5 |
Week 4 -Making thin sections and staining for electron microscopy. |
June 12 |
Week 5 -*Introduction to the transmission microscope (components of the TEM). |
June 19 |
Week 6 –*Quick dips, negative staining, SSEM and gold labeling. Using the digital and plate cameras, changing and developing film. |
June 27 |
Week 7 -*Digital Imaging, Electron microscopy, and Photo Shop. |
July 3 |
Week 8 –Break |
July 10 |
Week 9 -Making coated grids and use of the vacuum evaporator. |
July 17 |
Week 10 -Interpretation and critique of electron micrographs. Calibration and stigmation. |
July 24 |
Week 11 -*TEM class questions. Care and feeding of diamond knives. |
July 31 |
Week 12 -Individual help with finishing projects. |
*Lectures in Teaching Lab |
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